bv1000 bench mixer vortex mixer  (Benchmark Scientific)

Journal: Biophysical Reports

Article Title: Lipid loss and compositional change during preparation of simple two-component liposomes

doi: 10.1016/j.bpr.2024.100174

Figure Lengend Snippet: Fraction of total lipid remaining for postresuspension samples prepared with different vortexers (Vortex-Genie, BV1000, Fisherbrand). ( A ) A schematic of sample preparation. Postresuspension samples were prepared from 30:70 cholesterol/POPC lipid mixtures following the procedure listed in the materials and methods, using the vortexer brand as listed (30 s at max speed). Following sample collection, all samples were analyzed by HPLC-ELSD. ( B ) The fraction of total lipid remaining for each sample, calculated relative to the theoretical maximum moles of lipid that could be recovered if the entirety of the starting lipid material ended up in that sample (see ). Data shown are mean ± propagated error of 3 measurements (see for error calculations).

Article Snippet: Vortexers used included the Vortex-Genie (Scientific Industries, Bohemia, NY), the Fisherbrand Analog Vortex Mixer (Thermo Fisher Scientific, cat. no. 02-215-414), and the BV1000 Vortex Mixer (Benchmark Scientific, Edison, NJ).

Techniques: Sample Prep

Journal: Biophysical Reports

Article Title: Lipid loss and compositional change during preparation of simple two-component liposomes

doi: 10.1016/j.bpr.2024.100174

Figure Lengend Snippet: Fraction of total lipid remaining for postresuspension and postextrusion samples of liposomes prepared at different target lipid compositions. ( A ) A schematic of liposome preparation and sample collection for HPLC-ELSD analysis. Liposomes were prepared by the standard extrusion protocol at 4 different molar ratios of cholesterol/POPC = 30:70, 40:60, 50:50, 70:30 ( solid , dotted , striped data , respectively). Postresuspension samples were taken immediately after the resuspension of the lipid film, but before extrusion. The BV1000 or Fisherbrand vortexer (30 s at max speed) was used to resuspend all samples. Postextrusion samples were taken immediately after the extrusion step (see ). All samples were analyzed by HPLC-ELSD. ( B and C ) The fraction of total lipid remaining for the postresuspension and postextrusion samples, respectively. Each was calculated relative to the theoretical maximum moles of lipid that could be recovered if the entirety of the starting lipid material ended up in that sample (see ). Data shown are mean ± SD of 2 or 3 replicate samples.

Article Snippet: Vortexers used included the Vortex-Genie (Scientific Industries, Bohemia, NY), the Fisherbrand Analog Vortex Mixer (Thermo Fisher Scientific, cat. no. 02-215-414), and the BV1000 Vortex Mixer (Benchmark Scientific, Edison, NJ).

Techniques: Liposomes

Journal: Biophysical Reports

Article Title: Lipid loss and compositional change during preparation of simple two-component liposomes

doi: 10.1016/j.bpr.2024.100174

Figure Lengend Snippet: Deviation from expected mol percent of cholesterol ( green ) and POPC ( orange ) for postextrusion samples of liposomes prepared at different lipid compositions. Liposomes were prepared by the standard extrusion protocol at 4 different molar ratios of cholesterol/POPC = 30:70 ( A ), 40:60 ( B ), 50:50 ( C ) 70:30 ( D ). The BV1000 or Fisherbrand vortexer (30 s at max speed) was used to resuspend all samples before extrusion. Postextrusion samples were taken immediately after the extrusion step (see , and schematic in Fig. 6 A ), and analyzed by HPLC-ELSD. The expected mol percent is the mol percent in the parent lipid mixture before any manipulation. For each sample, data shown are the mean ± SD of 3 replicate measurements.

Article Snippet: Vortexers used included the Vortex-Genie (Scientific Industries, Bohemia, NY), the Fisherbrand Analog Vortex Mixer (Thermo Fisher Scientific, cat. no. 02-215-414), and the BV1000 Vortex Mixer (Benchmark Scientific, Edison, NJ).

Techniques: Liposomes

Journal: STAR Protocols

Article Title: Adverse outcome pathway-based analysis of liver steatosis in vitro using human liver cell lines

doi: 10.1016/j.xpro.2023.102500

Figure Lengend Snippet:

Article Snippet: Vortex mixer, e.g., BenchMixer Vortexer mixer , Benchmark Scientific , N/A.

Techniques: Recombinant, Modification, Spectroscopy, Saline, Transfection, Reverse Transcription, Software, Cell Culture, Suction Filtration, Membrane, Cell Counting, Inverted Microscopy, Transferring, Mass Measurement, Sterility, Pore Size, Spectrophotometry, cDNA Synthesis, Real-time Polymerase Chain Reaction